High-Sensitivity Spectrometer-Lisen Optics

The LiSpec-HS series employs stray light reduction technology and optimized optical path design, achieving measurement accuracy in transmittance and reflectance very close to that of large spectrophotometers. Its key advantages include low-noise circuitry, low jitter triggering, and compatibility with a variety of LiSen Optics accessories, such as light sources, reflection probes, fluorescence absorption cuvette holders, integrating spheres, collimating lenses, and diffuse reflectance standard boards, enabling the implementation of various spectral solutions. It also supports different slits, grating numbers, allowing for different wavelength ranges and optical resolutions. Additionally, this spectrometer supports various triggering methods, supports secondary development, and offers personalized, comprehensive customization services.

Technical Features

● Spectral range from 200 to 1100 nm, with the flexibility to change slits and use various optical accessories for expanded versatility.

● Utilizes back-illuminated BT-CCD/CMOS array detectors, offering high quantum efficiency in response to ultraviolet, visible, and near-infrared spectra, and a wide dynamic range.

● Unique stray light reduction technology and noise reduction circuit control technology contribute to high sensitivity and signal-to-noise ratio.

● Equipped with LiSpecView, a full-function spectral testing software, providing modules for transmittance and reflectance, absolute radiation, Raman, absorbance, laser power, and color measurements among others.

The LiSpec-HS series employs stray light reduction technology and optimized optical path design, achieving measurement accuracy in transmittance and reflectance very close to that of large spectrophotometers. Its key advantages include low-noise circuitry, low jitter triggering, and compatibility with a variety of LiSen Optics accessories, such as light sources, reflection probes, fluorescence absorption cuvette holders, integrating spheres, collimating lenses, and diffuse reflectance standard boards, enabling the implementation of various spectral solutions. It also supports different slits, grating numbers, allowing for different wavelength ranges and optical resolutions. Additionally, this spectrometer supports various triggering methods, supports secondary development, and offers personalized, comprehensive customization services.

Technical Features

● Spectral range from 200 to 1100 nm, with the flexibility to change slits and use various optical accessories for expanded versatility.

● Utilizes back-illuminated BT-CCD/CMOS array detectors, offering high quantum efficiency in response to ultraviolet, visible, and near-infrared spectra, and a wide dynamic range.

● Unique stray light reduction technology and noise reduction circuit control technology contribute to high sensitivity and signal-to-noise ratio.

● Equipped with LiSpecView, a full-function spectral testing software, providing modules for transmittance and reflectance, absolute radiation, Raman, absorbance, laser power, and color measurements among others.

Main Technical Indicators

Model	LiSpec-HS400	LiSpec-HS4000
Pic.
Spectral Range	200-1100nm	200-1100 nm
Optical Resolution (FWHM)	0.2–5 nm	0.2–5 nm
Wavelength Accuracy	≤±0.5 nm	≤±0.5 nm
Stray Light	< 0.1%	<0.3%
Detector	Area array 2048-pixel BT-CCD	Area array 2048-pixel BT-CCD
Signal-to-Noise Ratio	600:1	550:1
Dynamic Range	10,000:1	6,000:1
Dark Noise (RMS)	6 counts	11 counts
AD Conversion	16-bit, 0.5 MHz	16-bit, 15 MHz
Integration Time	6 ms–100 s	0.2 ms–65 s
Communication Interface	USB 2.0	USB 2.0 / 8Pin GPIO
I/O Interface	DB-15 interface: 1 serial signal, 3 × 485 signals, 1 external trigger for spectral acquisition, 1 power signal, 1 ground signal, 1 xenon lamp trigger signal, 5 digital level signals	8Pin GPIO
Power Supply	250 mA / 5 VDC, USB default power	300 mA / 5 VDC, USB default power
Operating Temperature	0-55°C	0–55°C
Dimensions	150 × 115 × 45 mm	110 × 86 × 35.4 mm

Spectrometer’s Resolution Selection Table

LiSpec-HS400
No.	Wavelength Range（nm）		Grating wavelength coverage range（nm）		Grating Lines (lines/mm)		Slit width (μm)
							25		50		100		200
							Resolution (nm)
1	200-1100		800		500g/mm		1.5		3		6		12
2	200-1100		700		600g/mm		1		2		4		8
3	200-1100		350		1200g/mm		0.5		1		2		4
4	200-1100		233		1800g/mm		0.35		0.7		1.4		2.8
LiSpec-HS4000
No.	Wavelength Range（nm）	Grating wavelength coverage range（nm）		Grating Lines (lines/mm)		Slit width (μm)
						10		25		50		100		200
						Resolution (nm)
1	200-1100	920		300 g/mm		1.5		2.5		4		8		15
2	200-1100	825		500 g/mm		1.2		1.5		2.5		7		9
3	200-1100	670		600 g/mm		1		1.5		2		3.5		7
4	200-1100	400		900 g/mm		0.8		1		1.3		2.5		5
5	200-1100	300		1000 g/mm		0.6		0.8		1.2		2.1		4
6	200-1100	220		1200 g/mm		0.6		0.7		1		2		3.5
7	200-920	250		1400 g/mm		0.4		0.6		0.9		1.5		3
8	200-380	200		1800 g/mm		0.3		0.5		0.6		1		1.8
9	200-540	140		2400 g/mm		0.3		0.4		0.5		0.9		1.6

Note: Specialized Wavelength Range Can be Customized

Main Technical Indicators

Model	LiSpec-HS400	LiSpec-HS4000
Pic.
Spectral Range	200-1100nm	200-1100 nm
Optical Resolution (FWHM)	0.2–5 nm	0.2–5 nm
Wavelength Accuracy	≤±0.5 nm	≤±0.5 nm
Stray Light	< 0.1%	<0.3%
Detector	Area array 2048-pixel BT-CCD	Area array 2048-pixel BT-CCD
Signal-to-Noise Ratio	600:1	550:1
Dynamic Range	10,000:1	6,000:1
Dark Noise (RMS)	6 counts	11 counts
AD Conversion	16-bit, 0.5 MHz	16-bit, 15 MHz
Integration Time	6 ms–100 s	0.2 ms–65 s
Communication Interface	USB 2.0	USB 2.0 / 8Pin GPIO
I/O Interface	DB-15 interface: 1 serial signal, 3 × 485 signals, 1 external trigger for spectral acquisition, 1 power signal, 1 ground signal, 1 xenon lamp trigger signal, 5 digital level signals	8Pin GPIO
Power Supply	250 mA / 5 VDC, USB default power	300 mA / 5 VDC, USB default power
Operating Temperature	0-55°C	0–55°C
Dimensions	150 × 115 × 45 mm	110 × 86 × 35.4 mm

Spectrometer’s Resolution Selection Table

LiSpec-HS400
No.	Wavelength Range（nm）		Grating wavelength coverage range（nm）		Grating Lines (lines/mm)		Slit width (μm)
							25		50		100		200
							Resolution (nm)
1	200-1100		800		500g/mm		1.5		3		6		12
2	200-1100		700		600g/mm		1		2		4		8
3	200-1100		350		1200g/mm		0.5		1		2		4
4	200-1100		233		1800g/mm		0.35		0.7		1.4		2.8
LiSpec-HS4000
No.	Wavelength Range（nm）	Grating wavelength coverage range（nm）		Grating Lines (lines/mm)		Slit width (μm)
						10		25		50		100		200
						Resolution (nm)
1	200-1100	920		300 g/mm		1.5		2.5		4		8		15
2	200-1100	825		500 g/mm		1.2		1.5		2.5		7		9
3	200-1100	670		600 g/mm		1		1.5		2		3.5		7
4	200-1100	400		900 g/mm		0.8		1		1.3		2.5		5
5	200-1100	300		1000 g/mm		0.6		0.8		1.2		2.1		4
6	200-1100	220		1200 g/mm		0.6		0.7		1		2		3.5
7	200-920	250		1400 g/mm		0.4		0.6		0.9		1.5		3
8	200-380	200		1800 g/mm		0.3		0.5		0.6		1		1.8
9	200-540	140		2400 g/mm		0.3		0.4		0.5		0.9		1.6

Note: Specialized Wavelength Range Can be Customized

Dimension (Unit: mm)

● LiSpec-HS400

● LiSpec-HS4000

Typical Application

● Raman Spectroscopy Measurement

The Raman measurement system mainly consists of a spectrometer, a laser, a Raman probe, and Raman identification spectrum analysis software. Raman scattering is primarily Stokes and anti-Stokes, with Stokes Raman scattering usually much stronger than anti-Stokes scattering. Raman spectrometers typically measure Stokes scattering. Commonly used Raman spectrometers include 532/785/1064 Raman spectrometers. Raman measurements are generally an order of magnitude weaker than fluorescence signals. For weak Raman signal measurements, Surface-Enhanced Raman Scattering (SERS) is employed to amplify the Raman signal. LiSen Optics' LiSpec-UV series of industrial high-speed spectrometers, with their high sensitivity and high signal-to-noise ratio, can be paired with lasers and Raman probes to measure weak spectral signals, widely used in food safety, chemical laboratories, biology, and medical optics for the determination and confirmation of substance composition. They are also applicable in forensics for drug detection and in the jewelry industry for gemstone identification.

● Color Measurement

The color of an object can be described by the CIE1976 (Lab*) color space. L* represents the brightness of the color, positive a* values represent red, negative a* values represent green, hue, and chroma. Similarly, positive b* values represent yellow, and negative b* values represent blue. Lab* values can be derived from the CIE tristimulus values X, Y, Z of the sample (object) and the tristimulus values Xn, Yn, Zn of the standard light source. The tristimulus values X, Y, Z of an object's color are obtained by multiplying the relative power P of the standard light source, the reflectance R (or transmittance T) of the object, and the CIE standard observer functions X_λ,y_λ,z_λ (for 2 or 10-degree fields). Integrating these values over the visible spectrum range (from 380 to 780 nm, at 5 nm intervals) yields the tristimulus values. Professional color measurement software can compare the measured Lab* values with reference colors to calculate the color difference（△E_lab,△L^*,△a^*,△b^*）.

● LED Color Measurement

● Radiation Measurement

Radiant light energy can be quantified as radiant flux, which is a standard measure of the energy radiated per second (W) from a light source. Radiation measurements generally require absolute radiometric calibration of the spectrometer system with a standard light source of known spectral energy distribution. This calibration allows for radiation measurements through quantifiable parameters. Radiation energy related to human vision (photometry) can obtain the spectral luminous efficiency functions defined by the CIE, which represent the average vision of observers. Therefore, radiometric parameters, photometric parameters, and colorimetric parameters are defined in radiation measurement. Radiometric parameters mainly include irradiance μW/cm², radiance µWatt/sr, radiant flux µWatt, and photon count µMol/s/m², µMol/m², µMol/s, and µMol. Photometric parameters include lumens, illuminance Lux, and intensity Candela. Colorimetric parameters include X, Y, Z, x, y, z, u, v, color temperature, CRI color rendering index, etc.

● Irradiance Measurement

● LED Color Measurement

● Absorbance Measurement

The method of measuring absorbance with a spectrometer involves passing parallel light of a certain wavelength through a flat parallel sample and detecting the light transmitted through the sample. Since a portion of the energy is absorbed by molecules in the sample, the intensity of the detected incident light is higher than that transmitted through the sample. Absorbance is widely used in spectral measurement techniques for liquids and gases for quantitative identification of substances or fingerprint authentication. It can also be integrated into industrial applications and tests of interest to customers. Using LiSen Optics' modular spectrometers, specific absorbance measurements can be selected based on different wavelength ranges and resolutions. In the lab or field, the entire optical measurement setup can be quickly configured. Based on the high-quality spectrometers from LiSen Optics, a flexible and easy-to-use combination can be made by choosing UV light sources, different optical path gas chambers, absorption cells, specific absorption path modules, and fiber probes, offering a variety of configuration options for different absorbance test

● Liquid Absorbance

Absorbance Measurement (Cuvette)

Absorbance Measurement (Fiber Probe)

● Gas Absorbance

● Thin Film Measurement

Thin film measurement systems are based on the principle of white light interferometry to determine the thickness of optical thin films. The thickness of the film is calculated through mathematical functions applied to the white light interference patterns. For a single-layer film, the physical thickness can be calculated if the refractive index (n) and extinction coefficient (k) of the film medium are known. The measurable film thickness ranges from 10 nm to 50 µm, with a resolution of up to 1 nm. Thin film measurement is applied in the semiconductor chip production industry, where it is necessary to monitor plasma etching and deposition processes. It can also be used in other fields requiring measurement of transparent film layers deposited on metal and glass substrates, such as transparent coatings on metal surfaces and glass substrates.

● Transmission/Reflection Spectral Measurement

With the booming industrial development, the quality control of the material characteristics has become increasingly strict. The technology of rapid and accurate transmission/reflection spectral measurements using fiber optic spectrometers is becoming more mature. Transmission/reflection spectral measurement is a basic method of spectral measurement, typically requiring equipment such as a spectrometer, light source, optical fiber, measurement stand, standard reference samples, and measurement software. To obtain better spectral data from different types of samples, these two basic modes may evolve into many more forms. Fiber optic spectrometers use optical fiber paths, overcoming the limitations of optical paths in instrument integration. LiSen Optics' fiber optic spectrometers are characterized by their small size, high stability, support for secondary software development, and a rich array of accessories, and have been successfully applied in the testing of industries such as glass and polymer materials. LiSen Optics provides users with spectral measurement equipment centered around spectrometers, and with these richly configured devices, various common spectral measurement systems can be easily set up.

Dimension (Unit: mm)

● LiSpec-HS400

● LiSpec-HS4000

Typical Application

● Raman Spectroscopy Measurement

The Raman measurement system mainly consists of a spectrometer, a laser, a Raman probe, and Raman identification spectrum analysis software. Raman scattering is primarily Stokes and anti-Stokes, with Stokes Raman scattering usually much stronger than anti-Stokes scattering. Raman spectrometers typically measure Stokes scattering. Commonly used Raman spectrometers include 532/785/1064 Raman spectrometers. Raman measurements are generally an order of magnitude weaker than fluorescence signals. For weak Raman signal measurements, Surface-Enhanced Raman Scattering (SERS) is employed to amplify the Raman signal. LiSen Optics' LiSpec-UV series of industrial high-speed spectrometers, with their high sensitivity and high signal-to-noise ratio, can be paired with lasers and Raman probes to measure weak spectral signals, widely used in food safety, chemical laboratories, biology, and medical optics for the determination and confirmation of substance composition. They are also applicable in forensics for drug detection and in the jewelry industry for gemstone identification.

● Color Measurement

The color of an object can be described by the CIE1976 (Lab*) color space. L* represents the brightness of the color, positive a* values represent red, negative a* values represent green, hue, and chroma. Similarly, positive b* values represent yellow, and negative b* values represent blue. Lab* values can be derived from the CIE tristimulus values X, Y, Z of the sample (object) and the tristimulus values Xn, Yn, Zn of the standard light source. The tristimulus values X, Y, Z of an object's color are obtained by multiplying the relative power P of the standard light source, the reflectance R (or transmittance T) of the object, and the CIE standard observer functions X_λ,y_λ,z_λ (for 2 or 10-degree fields). Integrating these values over the visible spectrum range (from 380 to 780 nm, at 5 nm intervals) yields the tristimulus values. Professional color measurement software can compare the measured Lab* values with reference colors to calculate the color difference（△E_lab,△L^*,△a^*,△b^*）.

● LED Color Measurement

● Radiation Measurement

Radiant light energy can be quantified as radiant flux, which is a standard measure of the energy radiated per second (W) from a light source. Radiation measurements generally require absolute radiometric calibration of the spectrometer system with a standard light source of known spectral energy distribution. This calibration allows for radiation measurements through quantifiable parameters. Radiation energy related to human vision (photometry) can obtain the spectral luminous efficiency functions defined by the CIE, which represent the average vision of observers. Therefore, radiometric parameters, photometric parameters, and colorimetric parameters are defined in radiation measurement. Radiometric parameters mainly include irradiance μW/cm², radiance µWatt/sr, radiant flux µWatt, and photon count µMol/s/m², µMol/m², µMol/s, and µMol. Photometric parameters include lumens, illuminance Lux, and intensity Candela. Colorimetric parameters include X, Y, Z, x, y, z, u, v, color temperature, CRI color rendering index, etc.

● Irradiance Measurement

● LED Color Measurement

● Absorbance Measurement

The method of measuring absorbance with a spectrometer involves passing parallel light of a certain wavelength through a flat parallel sample and detecting the light transmitted through the sample. Since a portion of the energy is absorbed by molecules in the sample, the intensity of the detected incident light is higher than that transmitted through the sample. Absorbance is widely used in spectral measurement techniques for liquids and gases for quantitative identification of substances or fingerprint authentication. It can also be integrated into industrial applications and tests of interest to customers. Using LiSen Optics' modular spectrometers, specific absorbance measurements can be selected based on different wavelength ranges and resolutions. In the lab or field, the entire optical measurement setup can be quickly configured. Based on the high-quality spectrometers from LiSen Optics, a flexible and easy-to-use combination can be made by choosing UV light sources, different optical path gas chambers, absorption cells, specific absorption path modules, and fiber probes, offering a variety of configuration options for different absorbance test

● Liquid Absorbance

Absorbance Measurement (Cuvette)

Absorbance Measurement (Fiber Probe)

● Gas Absorbance

● Thin Film Measurement

Thin film measurement systems are based on the principle of white light interferometry to determine the thickness of optical thin films. The thickness of the film is calculated through mathematical functions applied to the white light interference patterns. For a single-layer film, the physical thickness can be calculated if the refractive index (n) and extinction coefficient (k) of the film medium are known. The measurable film thickness ranges from 10 nm to 50 µm, with a resolution of up to 1 nm. Thin film measurement is applied in the semiconductor chip production industry, where it is necessary to monitor plasma etching and deposition processes. It can also be used in other fields requiring measurement of transparent film layers deposited on metal and glass substrates, such as transparent coatings on metal surfaces and glass substrates.

● Transmission/Reflection Spectral Measurement

With the booming industrial development, the quality control of the material characteristics has become increasingly strict. The technology of rapid and accurate transmission/reflection spectral measurements using fiber optic spectrometers is becoming more mature. Transmission/reflection spectral measurement is a basic method of spectral measurement, typically requiring equipment such as a spectrometer, light source, optical fiber, measurement stand, standard reference samples, and measurement software. To obtain better spectral data from different types of samples, these two basic modes may evolve into many more forms. Fiber optic spectrometers use optical fiber paths, overcoming the limitations of optical paths in instrument integration. LiSen Optics' fiber optic spectrometers are characterized by their small size, high stability, support for secondary software development, and a rich array of accessories, and have been successfully applied in the testing of industries such as glass and polymer materials. LiSen Optics provides users with spectral measurement equipment centered around spectrometers, and with these richly configured devices, various common spectral measurement systems can be easily set up.

● Reflectance Measurement

Reflectance Measurement（Probe）

Reflectance Measurement (Integrating Sphere)

● Transmittance Measurement

● Fluorescence Spectral Measurement

Fluorescent substances emit radiation with a certain spectral distribution under the radiation energy of specific wavelengths, usually scattering energy radiation in all directions. The fluorescence energy generated in fluorescence spectral measurement is less than the energy of the photons of the excitation light, only about 3% of the excitation light energy. It has high sensitivity, strong selectivity, requires small sample quantities, is simple to use, and is environmentally friendly. It is widely used in engineering applications such as monitoring food safety in food processing, fluorescence diagnosis of lesions in biomedical applications, exploration of oil minerals in geology, determination of soil mineral composition, and detection of trace elements in substances. LiSen Optics' fiber optic spectrometers feature interchangeable slits, selectable wavelength ranges, and resolution design, allowing customers to freely configure and match the fluorescence measurement system with suitable parameters according to their needs.

Fluorescence Measurement (Powder, Solid)

● Reflectance Measurement

Reflectance Measurement（Probe）

Reflectance Measurement (Integrating Sphere)

● Transmittance Measurement

● Fluorescence Spectral Measurement

Fluorescent substances emit radiation with a certain spectral distribution under the radiation energy of specific wavelengths, usually scattering energy radiation in all directions. The fluorescence energy generated in fluorescence spectral measurement is less than the energy of the photons of the excitation light, only about 3% of the excitation light energy. It has high sensitivity, strong selectivity, requires small sample quantities, is simple to use, and is environmentally friendly. It is widely used in engineering applications such as monitoring food safety in food processing, fluorescence diagnosis of lesions in biomedical applications, exploration of oil minerals in geology, determination of soil mineral composition, and detection of trace elements in substances. LiSen Optics' fiber optic spectrometers feature interchangeable slits, selectable wavelength ranges, and resolution design, allowing customers to freely configure and match the fluorescence measurement system with suitable parameters according to their needs.

Fluorescence Measurement (Powder, Solid)

High-Sensitivity Spectrometer

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